pflag tnks  (Addgene inc)

Journal: eLife

Article Title: Limited dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction

doi: 10.7554/eLife.55015

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , Tankyrase2 SAM domain (human) , Nai-Wen Chi (Addgene plasmid # 34691) , NCBI NP_079511.1 , residues 867–940.

Techniques: Expressing, Recombinant, CRISPR, DNA Sequencing, Western Blot, Derivative Assay, DNA Profiling, Flow Cytometry, Fluorescence, Microscopy, Control, Cell Culture, FLAG-tag, Modification, Plasmid Preparation, Activity Assay, Purification, Staining, Reporter Gene Assay, Imaging, Sedimentation, Software

Journal: eLife

Article Title: Limited dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction

doi: 10.7554/eLife.55015

Figure Lengend Snippet: To measure the relative expression of Dvl2 constructs, whole cell lysates of Dvl TKO cells transfected with the indicated Dvl2 construct were analyzed by SDS-PAGE and imaged as anti-Dvl2 immunoblots. Expression levels from each transient transfection were closely matched to endogenous levels (marked as End. Dvl2). The average relative expression level of each mutant relative to WT Dvl2, as quantified using a Li-COR Odyssey 3.0 scanner, is indicated below its label. For clarity, unrelated lanes from a single gel blot were excised from the image, denoted by a slight gap. Different blots are separated by large gaps in the figure. Dvl2 constructs are abbreviated as follows: DE = 60*DE*61, DC = 64*DC*65, EE >KK = E22K/E24K, EE >QQ = E22Q/E22Q, NG = E815N/E816G, NQ/NG = D793N/E794Q/E815N/E816G, T2SAM = Tankyrase2 SAM.

Article Snippet: Recombinant DNA reagent , Tankyrase2 SAM domain (human) , Nai-Wen Chi (Addgene plasmid # 34691) , NCBI NP_079511.1 , residues 867–940.

Techniques: Expressing, Construct, Transfection, SDS Page, Western Blot, Mutagenesis

Journal: eLife

Article Title: Limited dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction

doi: 10.7554/eLife.55015

Figure Lengend Snippet: DIX mutants affecting either the intra- (Y27D) or inter- (DC, DE) strand interactions (dark gray); replacement of DIX with wild-type or mutant DAX domains (pink); or addition of heterologous oligomerization domains (Sm1, light gray; Tankrase2 SAM, green) expressed at near-endogenous levels in Dvl triple knockout (TKO) HEK293 cells, as measured by TOPFLASH. Schematic diagrams of the constructs are shown to the left: the portion of Dvl C-terminal to the DIX domain is shown in black, the Dvl DIX domain in blue, the Axin1 DAX domain in pink, the Sm1 heptamerization domain in light gray, and the Tankyrase2 SAM domain in green. The red asterisks indicate 1) the DIX Y27D or the equivalent DAX Y760D head mutant that blocks oligomerization, or 2) mutated sites within DAX that increase filament-forming propensity (DAX NQ/NG , DAX NG ). Red pluses indicate residue insertions (DC, DE) that interfere with the DIX inter-strand interface.

Article Snippet: Recombinant DNA reagent , Tankyrase2 SAM domain (human) , Nai-Wen Chi (Addgene plasmid # 34691) , NCBI NP_079511.1 , residues 867–940.

Techniques: Mutagenesis, Triple Knockout, Construct, Residue

Journal: eLife

Article Title: Limited dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction

doi: 10.7554/eLife.55015

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , Tankyrase2 SAM domain (human) , Nai-Wen Chi (Addgene plasmid # 34691) , NCBI NP_079511.1 , residues 867–940.

Techniques: Expressing, Recombinant, CRISPR, DNA Sequencing, Western Blot, Derivative Assay, DNA Profiling, Flow Cytometry, Fluorescence, Microscopy, Control, Cell Culture, FLAG-tag, Modification, Plasmid Preparation, Activity Assay, Purification, Staining, Reporter Gene Assay, Imaging, Sedimentation, Software